miR-24-3p/FGFR3 Signaling as a Novel Axis Is Involved in Epithelial-Mesenchymal Transition and Regulates Lung Adenocarcinoma Progression

Figure 2

FGFR3 is a direct target of miR-24-3p. (a) miR-24-3p binding sites in 3-UTR of FGFR3 were predicted by TargetScan. The seed region of miR-24-3p and the recognition site in the FGFR3 3-UTR are shown in red. (b) qRT-PCR analysis of FGFR3 mRNA expression in A549 and H1299 cells transfected with either miR-NC or miR-24-3p mimics. (c) Expression of miR-24 in A549 and H1299 cells was detected by qRT-PCR. ns: not significant. (d) Cell lysates from H1299 cells that overexpressed miR-24-3p were analyzed for FGFR3, ERK1/2, and AKT content as well as the levels of the corresponding phosphorylated proteins. (e) The sequences of the WT and MUT FGFR3 3-UTR were used for the dual luciferase reporter construct. (f) The pMIR-REPORT vector and either FGFR3-WT or FGFR3-MUT plasmids were cotransfected with miR-24-3p or miR-NC mimics in H1299 cells, after which relative luciferase activity was detected. .

(a)

(b)

(c)

(d)

(e)

(f)