HLA-A2-Restricted Epitopes Identified from MTA1 Could Elicit Antigen-Specific Cytotoxic T Lymphocyte Response

Figure 4

Specific T cells induced by MTA1_(1–283) secrete IFN-γ and lyse target cells. PBMCs from healthy HLA-A02⁺ donors were isolated and stimulated with protein MTA1_(1–283) (at final concentrations of 0, 1, 5, 10, 30, and 50 μg/ml) in RPMI 1640 medium supplemented with 50 U/ml interleukin-2 and 10% FCS once a week for 21 days. On day 21, the induced T cells were collected; (a) IFN-γ secretion was assessed by the ELISPOT assay; and between two groups. (b) Cytotoxic activity was assessed by the LDH assay using SW620 cells (HLA-A2⁺, MTA1⁺) as the target cells. T cells induced by PBS were used as negative controls. , , and vs. the control group. Data represent means ± SD ().

(a)

(b)