HLA-A2-Restricted Epitopes Identified from MTA1 Could Elicit Antigen-Specific Cytotoxic T Lymphocyte Response

Table 1

The data of ESI–MS and HLA-A0201 binding affinity/stability properties of the peptides evaluated in this study.

Peptide	Sequence	NetCTL1.2 rank	Predicted IC50 (nM)	ESI–MS[M + H/Na]^+		FI^a	DC50^b
				Calculated	Observed
P22	YLIRRIEEL	1	4.9	1204.49	1204.6	2.08	>2 h
P57	ALADKHATL	5	69.6	939.4	939.5	1.56	>6 h
P109	FLSRQLESL	2	10.1	1092.1	1092.6	2.61	>6 h
P129	TLLNETESL	3	76	1019.51	1019.5	1.28	>6 h
P173	YQADITDLL	4	14.4	1051.94	1051.5	0.22	—
COX-2321-329	ILIGETIKI			999.6	1000.3	1.905	>6 h
HBcAg18–27	FLPSDFFPSV			1155.32	1155.6	1.21	>6 h

^aFI = [mean fluorescence intensity (MFI) of the peptide − MFI background]/[MFI background]. ^bDC₅₀: time (h) required for the loss of 50% of the HLA-A0201/peptide complexes stabilized at 0 h.