Suppression of Proinflammatory Cytokines and Mediators in LPS-Induced RAW 264.7 Macrophages by Stem Extract of Alternanthera sessilis via the Inhibition of the NF-κB Pathway

Figure 5

Effect of stem extract of A. sessilis on the protein expression of inflammatory enzymes, cyclooxygenase- (COX-) 2, inducible nitric oxide synthase (iNOS), inflammatory regulator, nuclear factor NF-κB p65, and inhibitor of κB (IκBα) on the macrophage induced with LPS and treated with various concentrations of A. sessilis stem extract (50, 100, and 200 μg/mL) for 24 h. Protein was extracted from the cells and furthered to electrophoresis, and protein expression was detected by Western blots according to its appropriate antibodies. The experiments were carried out in triplicate, and images shown are representatives of triplicates.