Research Article

Polysaccharides from Chinese Herbal Lycium barbarum Induced Systemic and Local Immune Responses in H22 Tumor-Bearing Mice

Figure 6

LBP treatment maintained cytotoxicity of lymphocytes from TDLN and spleen. H22 cells as target cells were labeled with CFSE before incubation with lymphocytes. The effector cells (lymphocytes) and target cells (H22 cells) were incubated for 24 h at a ratio of 50 : 1. After staining of PI, FCM was performed to calculate the percentage of PI+ H22 cells. (a) The percentage of H22 cells killed by lymphocytes from the spleen. (b) The percentage of H22 cells killed by lymphocytes from TDLN. Data are represented as mean ± SD, in each group. and versus model group. CFSE: carboxyfluorescein diacetate succinimidyl ester; PI: propidium iodide; FCM: flow cytometry; TDLN: tumor draining lymph node.
(a)
(b)