Immunotherapy with Native Molecule rather than Hypoallergenic Variant of Pru p 3, the Major Peach Allergen, Shows Beneficial Effects in Mice

Figure 5

SCIT with Pru p 3 or R/A Pru p 3 suppressed allergen-specific CD4⁺ T-cell proliferation. After the sensitization with Pru p 3, BALB/c mice received SCIT with 10 or 100 μg of Pru p 3 or R/A Pru p 3 and challenged with Pru p 3. Splenocytes from the mice were stained with CSFE and cultured in the presence of Pru p 3 for 96 hours. (a) Gating strategy in flow cytometry analysis for proliferation of (b) CD4⁺ T-cells and (c) CD8⁺ T-cells in the cultured splenocytes.

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(b)

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