Research Article
Mycobacterium tuberculosis Protein Rv3841 Activates Dendritic Cells and Contributes to a T Helper 1 Immune Response
Figure 4
Rv3841-treated DCs stimulate T-cells to produce Th1 cytokines. (a, b) Transgenic OVA-specific CD4+ T-cells were isolated using MACS from OT-I and OT-II mouse splenocytes, were stained with CFSE, and were cocultured for 96 h with DCs that had been treated with Rv3841 (10 μg/mL) or LPS (100 ng/mL) and then pulsed with OVA257–264 (1 μg/mL) or OVA323–339 (1 μg/mL) to produce OVA-specific CD4+ T-cells. (a) The proliferation of OT-I T-cells was then assessed by flow cytometry. T-cells alone or T-cells cocultured with untreated DCs served as the controls. Representative histograms from three independent experiments are shown. Culture supernatants were harvested after 96 h, and the IFN-γ, IL-2, and IL-4 concentrations were determined by ELISAs. The mean values ± SD () are shown; or : a significant difference of treatment groups from the appropriate controls (T-cells + OVA257–264-pulsed DCs or T-cells + OVA323–339-pulsed DCs), as determined by one-way ANOVA. Treatments without a significant effect are indicated by “n.s.”
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