Research Article

Mycobacterium tuberculosis Protein Rv3841 Activates Dendritic Cells and Contributes to a T Helper 1 Immune Response

Figure 7

T-cells activated by Rv3841-treated DCs inhibit intracellular Mtb growth. Splenic T-cells or T-cells from Mtb-infected mice activated by unstimulated DCs, LPS-stimulated DCs, Ag85B-stimulated DCs, or Rv3841-stimulated DCs at a DC : T-cell ratio of 1 : 10 for 3 days were cocultured with BMDMs infected with Mtb. (a) Intracellular Mtb growth in BMDMs was determined at time point 0 (day 0) and 3 days after culturing with T-cells or without T-cells (control). (b) The NO and IFN-γ levels in culture supernatants were determined. The data are shown as mean ± SD (); , , or : a significant difference of BMDMs cocultured with T-cells from control BMDMs. n.s.: no significant difference.
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