Leaf-Encapsulated Vaccines: Agroinfiltration and Transient Expression of the Antigen Staphylococcal Endotoxin B in Radish Leaves

Figure 1

Transient expression of agroinfiltrated GUS in Arabidopsis, lettuce, and radish leaves. Transient encapsulation of GUS in leaves of three different plants. In an effort to transiently encapsulate GUS, leaves of Arabidopsis thaliana, the first selected to decipher its genome sequences, and two edible crops (lettuce (Lactuca sativa) and radish (Raphanw sativus L.)) were infiltrated with A. tumefaciens (LBA4404 strains, 5 × 10⁷ CFU) transforming a 35S::GUS construct (pBI121). Plant leaves infiltrated with nontransformed LBA4404 cells (control, 5 × 10⁷ CFU) served as negative controls. Dotted circles indicate locations of syringe infiltration with A. tumefaciens. GUS was detected using a histochemical staining procedure. Blue-stained areas indicate GUS activity. Bar = 6 mm.