The Imbalance between Foxp3⁺Tregs and Th1/Th17/Th22 Cells in Patients with Newly Diagnosed Autoimmune Hepatitis

Figure 6

Flow cytometry of Treg/Th1/Th17/Th22 cells in SMNCs and ELISA analysis of hepatic levels of IL-10, IFN-γ, IL-17A, and IL-22 in EAH mice. Ten mice (5 from the EAH group and 5 from the control group) were killed at each time point (1, 7, 14, and 28 days). (a) Flow cytometry analysis of Tregs and Th1, Th17, and Th22 cells from the spleen on the 14th day. (b–e) Percentages of Tregs and Th1, Th17, and Th22 cells at each time point from EAH and control mice were analyzed by FACS. (f–i) Liver IL-10, IFN-γ, IL-17A, and IL-22 levels at each time point from EAH and control mice were used for ELISA. The horizontal lines indicate the mean values of the different groups. and .

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