March1 E3 Ubiquitin Ligase Modulates Features of Allergic Asthma in an Ovalbumin-Induced Mouse Model of Lung Inflammation

Figure 3

March1 deficiency is associated with less IFN-γ-, IL-5-, and IL-13-producing Th cells in the ovalbumin-allergic model. Lung immune cells were isolated by digestion and stained for surface CD45 and CD4 (a). Then, cells were permeabilized and stained for IFN-γ (b), IL-5 (c), and IL-13 (e). Gating strategy of lung cell subpopulations (a), IFN-γ-producing CD4 cells (b), IL-5-producing CD4 cells (c), ratio of IL-5/IFN-γ CD4 T cells (d), and IL-13-producing CD4 cells (e); all normalized and expressed as % of CD45⁺ immune cells. , , (trend). Data are pooled from 3 independent experiments and presented in means with SEM (b, c) or medians with interquartile (d, e); –8/group.

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