March1 E3 Ubiquitin Ligase Modulates Features of Allergic Asthma in an Ovalbumin-Induced Mouse Model of Lung Inflammation

Table 1

March1 does not affect eosinophilic airway inflammation but induces neutrophilic inflammation in the airways: Total inflammatory cells in bronchoalveolar lavage (BAL) fluid were counted using hemocytometer and trypan blue exclusion method. Differential cell count in BAL: Differential cell count was performed on BAL cells adhered to slides and stained with HEMA-3. For each sample, 200–300 cells were counted under the microscope (40x), and their percentage was multiplied by the total cell count to extrapolate absolute differential count. and denote statistically significant; (trend); N.S.: non-ignificant. Data are presented as median (nonparametric data) and pooled from 5 independent experiments. –12/group.

	WT median cells ×10^4		value WT sal-ova versus ova-ova	March1^−/− median cells ×10^4		value March1^−/− sal-ova versus ova-ova	value ova-ova WT versus ova-ova March1^−/−
	sal-ova	ova-ova		sal-ova	ova-ova
Total cells/mouse BAL	6	15.9	0.09#	4	15.7	<0.001	N.S.
Mono/macs	5.16	4.9	N.S.	3.7	4.7	N.S.	N.S.
Lymphocytes	0	0.24	0.06#	0.01	0.28	0.06#	N.S.
Neutrophils	0	0.01	N.S.	0	0.27	0.02	N.S.
Eosinophils	0	1.9	<0.001	0.07	5.8	<0.001	N.S.