Stimulation of Dectin-1 and Dectin-2 during Parenteral Immunization, but Not Mincle, Induces Secretory IgA in Intestinal Mucosa

Figure 2

Response of bone marrow-derived dendritic cells to Mincle, Dectin-1, and Dectin-2 agonists. Cells were harvested on day 8 of culture, seeded in 24-well plates (2 × 10⁵ cells/well), and incubated for 24 h with 10 μg/mL of Mincle, Dectin-1, and Dectin-2 agonists. (a) Expression of the maturation markers CD40, CD80, and CD86 was assessed by flow cytometry of 10⁶ CD11c⁺ MHCII⁺ cells. Data are mean fluorescence fold increase over PBS-treated cells from two independent experiments with triplicates. Means were compared using the two-tailed unpaired Mann–Whitney t-test. Error bars indicate standard deviations. ^#; ^##; ^### versus PBS-treated cells. ; ; between Mincle-, Dectin-1-, and Dectin-2-stimulated cells. (b) Cytokines were quantified by bead-based immunoassay in media collected 24 h after addition of agonists. Data are mean fold change relative to PBS-treated cells in three independent experiments with duplicates. Data were compared using the two-tailed unpaired Mann–Whitney t-test. Error bars indicate standard deviations. ^#; ^##; ^### versus PBS-treated cells. ; ; between Mincle-, Dectin-1-, and Dectin-2-stimulated cells.

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