Stimulation of Dectin-1 and Dectin-2 during Parenteral Immunization, but Not Mincle, Induces Secretory IgA in Intestinal Mucosa
Cytokine and chemokine expression in murine splenic mononuclear cells after Mincle, Dectin-1, and Dectin-2 stimulation. Splenic mononuclear cells were isolated from C57/Bl6 mice, seeded in 96-well plates at 2 × 105 cells/well, and stimulated for 48 h with 10 μg/mL Mincle, Dectin-1, and Dectin-2 agonists. Cytokines were quantified in media by bead-based immunoassay. Data are mean fold change relative to PBS-treated cells in three independent experiments with duplicates, and error bars indicate standard deviations. Data were compared using the two-tailed unpaired Mann–Whitney t-test. #;##;### versus PBS-treated cells. ;; between Mincle-, Dectin-1-, and Dectin-2-stimulated cells.