Stimulation of Dectin-1 and Dectin-2 during Parenteral Immunization, but Not Mincle, Induces Secretory IgA in Intestinal Mucosa
Dectin-1 and Dectin-2 agonists induce production of antigen-specific IgA in the small intestine. Mice were immunized twice by subcutaneous injection of ovalbumin alone or in combination with TDB, curdlan, or furfurman. Naïve mice were used as negative control. IgA titers were quantified by ELISA in (a) intestinal and (b) bronchoalveolar lavages 14 days after the second immunization. Data are individual log2 antibody titers from two independent experiments (5 mice per group per experiment), with geometric mean and 95% confidence interval indicated. ; relative to control group by Mann–Whitney t-test.