Proteomic Identification of Heat Shock-Induced Danger Signals in a Melanoma Cell Lysate Used in Dendritic Cell-Based Cancer Immunotherapy

Figure 1

The HS conditioning of TRIMEL melanoma cells contributes to its in vitro DC maturation capacity. Representative density plots (a) and statistical quantification (b) of the DC-associated marker expression MHCI, MHCII, and CD80 in primary human cytokine-activated monocytes stimulated with TRIMEL (HS), or with the same lysate generated without heat shock conditioning (no-HS) (100 μg/mL) or without lysate (unstimulated (Unst)). (b) The quantification of the maturation marker expression considered the % positive cells, the geometric mean fluorescence intensity (gMFI) of the positive cells, and the integrated MFI (iMFI: % positive cells × gMFI of positive cells/100). The expression of surface markers was assessed by flow cytometry (CD11c + cells were gated). Data represent three independent experiments with PBMC derived from three different stage IV MM patients. (c) Bars indicate the average fold induction and standard deviation (SD) of the iMFI of DC markers relative to monocytes stimulated with no-HS lysate. and .

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