Proteomic Identification of Heat Shock-Induced Danger Signals in a Melanoma Cell Lysate Used in Dendritic Cell-Based Cancer Immunotherapy

Figure 3

Expression profiles of selected proteins from TRIMEL and regulated by HS treatment. (a) Heat map with hierarchical clustering of proteins differentially expressed between TRIMEL (HS) and non-HS samples using a cutoff at . Protein names are displayed on the right and below is depicted an augmented section of the 18 HS-overexpressed proteins. Red, overexpressed; blue, underexpressed; and white, no change. The color-coded scale is indicated at the top of the chart. (b) Log-transformed relative protein expression of the 18 proteins regulated by HS treatment. The text and table only refer to 17 proteins because in one case a protein group was identified (RPS27A, UBB, UBC, UBA52, and UBBP4). It means the set of peptides matches all these five proteins and we cannot distinguish them here.

(a)

(b)