|
Gene | Gene transfer vector | Signaling components | Cell type | Target | Gene transfer efficiency | Ref |
|
CD19 | mRNA electroporation | CD3ζ and 4-1BB | Primary NK cells | B cell malignancies | >95% cells | Carlsten et al. [178] |
EGFR | Lentivirus | CD3ζ and CD28 | NK-92 and NKL | Glioblastoma | N/A | Han et al. [80] |
CD19, antimesothelin | Sleeping beauty transposon | CD3ζ, CD28, and 4-1BB | iPSC-derived NK cells | B cell malignancies, ovarian cancer | N/A | Ni et al. [179] |
CD19 and IL-15 with iC9 suicide gene | Retrovirus | CD3ζ and CD28 | Cord blood NK cells | B cell malignancies | >80% | Liu et al. [66] |
ROR1 | mRNA electroporation | 4-1BB | Peripheral blood NK cells | Neuroblastoma and sarcoma | 70% cells | Park et al. [180] |
CD22 and CD19-ENG | Retrovirus | CD28 and 4-1BB | Peripheral blood NK cells | B cell malignancies | 70–80% of CD22-CARs, and ~50% bispecific CD19-T cell engagers | Velasquez et al. [181] |
CD19 | VGV-pseudotyped retrovirus | CD28-CD3ζ or CD137-CD3ζ | NK-92 | B cell malignancies | N/A | Oelsner et al. [182] |
CD123 | α-Retroviral SIN | CD28 and 4-1BB | Peripheral blood NK cells | Acute myeloid leukemia | 22.9% on day 3; 11.9% on day 9 | Klöß et al. [183] |
CD20 | pLXSN retrovirus | CD3ζ | NK-92 | B cell malignancies (in vitro) | 93.8–96.3% | Müller et al. [184] |
CS1 | PCDH lentivirus | CD3ζ and CD28 | NK-92 and NKL | Multiple myeloma | >98% (NK-92), >95% (NKL) | Chu et al. [185] |
CD4 | Lentivirus | CD3ζ, CD28, and 4-1BB | NK-92 | T cell lymphoma | >85% | Pinz et al. [186] |
PSCA | Self-inactivating pHATtrick lentivirus | CD3ζ, CD28, and DAP12 | Peripheral blood NK cells | Prostate cancer stem cells | ~50% | Töpfer et al. [74] |
NKp44 | Retrovirus | CD3ζ and CD28 | Peripheral blood NK cells | Enhanced cytotoxicity | N/A | Kasahara et al. [187] |
CD19 and HER2 | pCCW lentivirus | CD3ζ, CD28, and 4-1BB | NK-92 | Solid tumors | N/A | Siegler et al. [188] |
CD20 | mRNA nucleofection | CD3ζ and 4-1BB | Peripheral blood NK cells | Pediatric Burkitt lymphoma | N/A | Chu et al. [189, 190] |
CD138 | Lentivirus | CD3ζ (with CD8α) | NK-92MI | Multiple myeloma | >95% | Jiang et al. [130] |
ErbB2 | Lentivirus | CD3ζ and CD28 | NK-92 | Glioblastoma | N/A | Steinbach et al. [191] |
GD2 | pLXSN retrovirus | CD3ζ | NK-92 | Neuroblastoma | N/A | Esser et al. [192] |
CD33 | Electroporation | CD3ζ | YT | Acute myeloid leukemia | 90% (after enrichment) | Schirrmann and Pecher [193] |
NKG2D | Retrovirus | CD3ζ and DAP10 | Peripheral blood NK cells | T-cell ALL, B-cell ALL, osteosarcoma, prostate carcinoma, rhabdomyosarcoma, neuroblastoma, Ewing sarcoma, colon carcinoma, gastric carcinoma, lung squamous cell carcinoma, hepatoma, and breast carcinoma (in vitro), osteosarcoma (in vivo) | 80% (range 67–96%) | Chang et al. [194] |
EGFR | Lentivirus | CD3ζ and CD28 | NK-92 | Breast cancer | 39.4% | Chen et al. [195] |
HER-2 | Retrovirus | CD3ζ and CD28 | Peripheral blood NK cells | Ovarian and breast cancer cell lines | 55 ± 11% | Kruschinski et al. [131] |
CD19 | Trogocytosis | CD3ζ and 4-1BB | Peripheral blood NK cells | B-ALL cell lines | 47% upon coculture with donor cells | Cho et al. [161] |
TRAIL-receptor 1 | Retrovirus | CD3ζ, 4-1BB, and CD28 | KHYG-1 | Colo205, Daudi, K562 cells (in vitro) | N/A | Kobayashi et al. [196] |
GPC3 | Lentivirus | CD8, CD28, and CD3ζ | NK-92 and peripheral blood NK cells | Hepatocellular carcinoma | ~35% (primary NK cells) | Yu et al. [197] |
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