Anti-ICOSL New Antigen Receptor Domains Inhibit T Cell Proliferation and Reduce the Development of Inflammation in the Collagen-Induced Mouse Model of Rheumatoid Arthritis

Figure 6

Clinical scores and histopathology sections from the CIA study CIA study results. (a, b) Average clinical scores for each experimental group of 10 animals measured twice weekly for the duration of study. A collagen boost was given at day 20 and dosing of test samples at 15 mg/kg at days 19, 21, 23, and 25. Data shown as an average clinical and comparisons were made using the Mann-Whitney test. (c) Analysis of the ankle RA scores from the left hind joint from each animal: the level of inflammation, pannus formation, cartilage damage, bone resorption, and periosteal change was measured, and each of these parameters was allocated a score from 1 to 5. The summarised histopathology scores were plotted. (d) Left hind paws from each experimental animal were sectioned and stained with H&E. (i) A1-Fc, (ii) CC3-Fc, (iii) 2V-Fc, (iv) HK5.3, (v) IgG2a, (vi) dexamethasone, and (vii) vehicle control. Black arrow: an example region of bone erosion; grey arrow: an example region of bone resorption; white arrow: cellular infiltrate; yellow arrow: pannus formation. The size bar represents 2 mm.

(a)

(b)

(c)

(d)