A DNA-Methylated Sight on Autoimmune Inflammation Network across RA, pSS, and SLE

Figure 1

Workflow of methodology applied in this study. Step 1: the collection of methylated chip datasets of RA, pSS, and SLE and the conduction of quality control and normalization. Step 2: the generation of Cytokine-Chips. Step 3: the investigation of cytokine-cytokine interactome hotspots. Step 4: the integrative analysis for identifying differences and similarities on DNA methylation status across the 3 AIDs. Step 5: the GO and KEGG enrichment analysis of cytokine genes with aberrant methylation. QC, quality control; DMPs, differential methylated positions; DMRs, differential methylation regions; 450K Chip: Illumina Infinium HumanMethylation450 BeadChip.