Tag7 (PGLYRP1) Can Induce an Emergence of the CD3+CD4+CD25+CD127+ Cells with Antitumor Activity

Figure 3

Flow cytometry of the total PBMC pool on day 6 of incubation with Tag7 (a), the Tag7-positive subpopulation isolated via magnetic separation (b), and the Tag7-negative subpopulation (c) stained with the CD25-FITC, CD127-PE, and CD4-TC antibodies. The left panel shows the staining with the anti-CD25 and anti-CD4 antibodies, and the right panel shows the staining of the CD4+CD25^high subpopulation from the left panel with the anti-CD127 and anti-CD25 antibodies.

(a)

(b)

(c)