Research Article

Luteolin Induces Apoptosis and Autophagy in Mouse Macrophage ANA-1 Cells via the Bcl-2 Pathway

Figure 4

Luteolin increased the ANA-1 macrophage autophagosome using immune fluorescence combined with CLSM. (a) Immunofluorescence staining of LC3.The ANA-1 cells were seeded on glass cover slips in 24-well plates with luteolin for 48 h. The fluorescence images were captured with a confocal microscopy. Magnification, ×1000. (b) Average intracellular autophagosome in macrophages treated with luteolin for 48 h. The values are given as mean ± SEM. and versus the control.
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