Activation of PAR4 Upregulates p16 through Inhibition of DNMT1 and HDAC2 Expression via MAPK Signals in Esophageal Squamous Cell Carcinoma Cells

Figure 4

Silencing PAR4 induces DNMT1, HDAC2, and p16 expression in ESCC cells. (a) ESCC cells were transiently transfected with PAR4-siRNA1 or PAR4-siRNA2. Western blotting was performed to examine the expression of DNMT1, HDAC2, p16, and PAR4 at protein levels after transfection with PAR4-siRNA for 72 h. (b) qRT-PCR was performed to examine the expression of DNMT1, HDAC2, and p16 expression at mRNA levels after transfection with PAR4-siRNA for 72 h. The results were calculated by normalizing to β-actin in the same sample with the ΔCt method. The data are expressed as the mean ± SEM (). versus controls.

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