Activation of PAR4 Upregulates p16 through Inhibition of DNMT1 and HDAC2 Expression via MAPK Signals in Esophageal Squamous Cell Carcinoma Cells
Silencing PAR4 induces DNMT1, HDAC2, and p16 expression in ESCC cells. (a) ESCC cells were transiently transfected with PAR4-siRNA1 or PAR4-siRNA2. Western blotting was performed to examine the expression of DNMT1, HDAC2, p16, and PAR4 at protein levels after transfection with PAR4-siRNA for 72 h. (b) qRT-PCR was performed to examine the expression of DNMT1, HDAC2, and p16 expression at mRNA levels after transfection with PAR4-siRNA for 72 h. The results were calculated by normalizing to β-actin in the same sample with the ΔCt method. The data are expressed as the mean ± SEM (). versus controls.
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