M1-Polarized Macrophages Promote Self-Renewing Phenotype of Hepatic Progenitor Cells with Jagged1-Notch Signalling Involved: Relevance in Primary Sclerosing Cholangitis

Figure 2

M1 macrophages promote a self-renewing phenotype of HPCs in a coculture system. (a) HPCs were cocultured (24 hours) with M0, M1, or M2 macrophages from EGFP transgenic C57BL/6 mice. After flow cytometry analysis, CD44⁺CD324⁺ and CD44⁺CD326⁺ cell fractions of HPC are highlighted by scatter plots. (b) Flow cytometry data were calculated and represented. vs HPC, vs M0 macrophages cocultured with HPC, and vs M2 macrophages cocultured with HPC.

(a)

(b)