DNA Methyltransferase Inhibition Promotes Th1 Polarization in Human CD4⁺CD25^high FOXP3⁺ Regulatory T Cells but Does Not Affect Their Suppressive Capacity

Figure 2

Effect of DAC on proliferation and suppressive capacity of CD4⁺ Treg. (a) Representative dot plots, showing the expression of Ki67 in αCD3/αCD28⁺ 100U rIL-2 stimulated FACS-sorted Tconv and Treg at day 8 of culture. (b) Cumulative data showing the percentage of Ki67-positive cells in Tconv and Treg stimulated in the presence or absence of 1 μM DAC for 8 days (). (c) Effect of DAC on the suppressive capacity of CD4⁺ Treg. Flow cytometry of a CFSE-based suppression assay of αCD3/αCD28⁺ 100 U IL-2-stimulated Treg that were cultured for 8 days in the presence or absence of 1 μM DAC. (d) Cumulative data showing the percentage dividing responder cells in the presence of Treg with or without DAC. . are shown. (b, c) Wilcoxon signed-rank test, 2-tailed,

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