DNA Methyltransferase Inhibition Promotes Th1 Polarization in Human CD4⁺CD25^high FOXP3⁺ Regulatory T Cells but Does Not Affect Their Suppressive Capacity

Figure 5

Effect of DAC on cytokine-producing potential of CD4⁺ Treg under proinflammatory conditions. Flow cytometric analysis of αCD3/αCD28⁺ 100U IL-2 + 50 ng/mL IL-1β-stimulated FACS-sorted Treg and Tconv on day 8 of culture. The cytokine-producing potential was analyzed following 4 h stimulation with PMA/ionomycin in the presence of Brefeldin A. Cumulative data on IFNγ-, IL-17a-, and IL-10-producing Treg and Tconv that were cultured in the presence or absence of 1 μM DAC. are shown. , Wilcoxon signed-rank test, 2-tailed.