DNA Methyltransferase Inhibition Promotes Th1 Polarization in Human CD4⁺CD25^high FOXP3⁺ Regulatory T Cells but Does Not Affect Their Suppressive Capacity

Figure 6

In vivo effect of Vidaza treatment on FOXP3 expression and cytokine expression by CD4 T cells. Flow cytometry of isolated PBMCs obtained from Vidaza-treated patients with hematological malignancies. (a) Gating strategy used for FOXP3 analyses. (b) Difference (Δ) in percentage FOXP3⁺ cells and MFI after 7 days (short term) () and after 5 cycles (long term) () of Vidaza. (c) Gating strategy used for cytokine analysis in CD4⁺ and CD4⁺FOXP3⁺ T cells after 4 h PMA/ionomycin stimulation in the presence of Brefeldin A. (d) Difference (Δ) in percentage of IFNγ, IL-17A, and IL-10 in CD4⁺ T cells and CD4⁺FOXP3⁺ Treg after 7 days and 5 cycles of Vidaza. Paired t-test, no significant differences.

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