Research Article
Variation in Expression of Inflammation-Related Signaling Molecules with Profibrotic and Antifibrotic Effects in Cutaneous and Oral Mucosa Scars
Table 1
Primary antibodies: technical specifications.
| Primary antibody | Source | Clone | Epitope retrieval | Dilution | Incubation period (min) |
| TGFbeta1 | ABCAM | Policlonal | HIER pH 6 | 8/200 | 60 | TGFbeta2 | ABCAM | ab36495 | HIER pH 6 | 0.5/250 | 60 | TGFbeta3 | ABCAM | Policlonal | HIER pH 6 | 8/200 | 60 | TNFalpha | ABCAM | Policlonal | HIER pH 6 | 1/250 | 60 | PDGF BB | ABCAM | Policlonal | HIER pH 6 | 1/250 | 30 | FGF1 | ABCAM | Policlonal | HIER pH 6 | 1/200 | 60 |
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HIER = heat-induced epitope retrieval. |