The Solute Carrier Transporter SLC15A3 Participates in Antiviral Innate Immune Responses against Herpes Simplex Virus-1

Figure 3

SLC15A3 protects host cells from HSV-1 infection. (a) Western blot analysis of SLC15A3 protein in 293T after overexpression. (b) PCR analysis of HSV-1 gD gene abundance in SLC15A3 overexpressed 293T cells. (c) Plaque assays of HSV-1 infectious particles produced in 293T transfected with empty vector and SLC15A3. (d) PCR analysis of SLC15A3 and HSV-1 gD gene expression in 293T transfected with scrambled siRNA and SLC15A3 siRNA for 24 h then addition of HSV-1 for 24 h. (e) Plaque assays of HSV-1 infectious particles produced in 293T. 293 T cells were transfected with scrambled siRNA and SLC15A3 siRNA for 24 h then addition of HSV-1 for 24 h. , (unpaired -test). Data are representative of three experiments with similar results (mean ± sd in (b), (c), (d), (e)).

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