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Journal of Immunology Research
Volume 2018, Article ID 5379085, 12 pages
Research Article

Aspergillus fumigatus Infection-Induced Neutrophil Recruitment and Location in the Conducting Airway of Immunocompetent, Neutropenic, and Immunosuppressed Mice

1Laboratory of Cell Interactions, Department of Immunology, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St. 16/10, Moscow 117997, Russia
2Laboratory for Advanced Studies of Membrane Proteins, Moscow Institute of Physics and Technology, 9 Institutskiy Per., Dolgoprudny 141701, Russia
3Institute of Complex Systems 4 (ICS-4: Cellular Biophysics), Forschungszentrum Jülich, 52425 Jülich, Germany
4Institute of Complex Systems 6 (ICS-6: Structural Biochemistry), Forschungszentrum Jülich, 52425 Jülich, Germany
5Université Grenoble Alpes, CEA, CNRS, IBS, 38000 Grenoble, France

Correspondence should be addressed to Marina A. Shevchenko; moc.liamg@hcvehsm and Valentin I. Borshchevskiy; ude.hcetsyhp@iv.yiksvehchsrob

Received 25 August 2017; Revised 5 November 2017; Accepted 22 November 2017; Published 18 January 2018

Academic Editor: Elzbieta Kolaczkowska

Copyright © 2018 Marina A. Shevchenko et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Susceptibility to fungal infection is commonly associated with impaired neutrophil responses. To study the mechanisms underlying this association, we investigated neutrophil recruitment to the conducting airway wall after Aspergillus fumigatus conidium inhalation in mouse models of drug-induced immunosuppression and antibody-mediated neutrophil depletion (neutropenia) by performing three-dimensional confocal laser-scanning microscopy of whole-mount primary bronchus specimens. Actin staining enabled visualization of the epithelial and smooth muscle layers that mark the airway wall. Gr-1+ or Ly6G+ neutrophils located between the epithelium and smooth muscles were considered airway wall neutrophils. The number of airway wall neutrophils for immunocompetent, immunosuppressed, and neutropenic mice before and 6 h after A. fumigatus infection were analyzed and compared. Our results show that the number of conducting airway wall neutrophils in immunocompetent mice significantly increased upon inflammation, while a dramatic reduction in this number was observed following immunosuppression and neutropenia. Interestingly, a slight increase in the infiltration of neutrophils into the airway wall was detected as a result of infection, even in immunosuppressed and neutropenic mice. Taken together, these data indicate that neutrophils are present in intact conducting airway walls and the number elevates upon A. fumigatus infection. Conducting airway wall neutrophils are affected by both neutropenia and immunosuppression.