Development of a Cell-Based High-Throughput Screening Assay to Identify Porcine Host Defense Peptide-Inducing Compounds

Figure 2

Strictly standardized mean difference (SSMD) values of individual compounds in the primary screening of an Epigenetics Screening Library (compound #1-148) and two natural product libraries (compound #149-732). The IPEC-J2/PBD3-luc luciferase reporter cell line was stimulated with 20 μM of each compound in the Epigenetics Screening Library or 20 μg/ml of each compound in the natural product libraries for 24 h, followed by the luciferase assay. The alamarBlue Dye was added 4 h before the luciferase assay to measure cell viability. The luciferase activity of each compound was normalized to cell viability before the SSMD value was calculated.