Development of a Cell-Based High-Throughput Screening Assay to Identify Porcine Host Defense Peptide-Inducing Compounds

Figure 3

Concentration-dependent changes in the luciferase activity in stable IPEC-J2/PBD3-luc luciferase reporter cells in response to different concentrations of 43 unique hits identified in the primary screening. For the compounds in the Epigenetics Screen Library, the three concentrations of each compound were 5, 20, and 80 μM, whereas the compounds in the natural product libraries were applied at 5, 20, and 80 μg/ml in duplicate for 24 h. Sodium butyrate at 16 mM was used as the positive control. Note that apicidin and HC toxin were identified from both libraries. The results are means ± SEM of three independent experiments.