Development of a Cell-Based High-Throughput Screening Assay to Identify Porcine Host Defense Peptide-Inducing Compounds

Figure 6

Augmentation of the antibacterial activity of porcine 3D4/31 macrophages by the selected HDAC inhibitors. Porcine 3D4/31 cells were left unstimulated or stimulated with 20 μM SAHA, 20 μM apicidin, or 5 μM HC toxin for 24 h. Sodium butyrate (16 mM) was used as a positive control. Cell lysates were then incubated with E. coli ATCC 25922 (a) or S. aureus ATCC 43300 (b) at 37°C for 3, 6, and 9 h. The bacterial turbidity was measured at OD₆₀₀. The results are means ± SEM of 2–3 independent experiments. Two-way analysis of variance (ANOVA), followed by Dunnett’s multiple comparison test, was performed. , , and (relative to the control at each time point).