Acer palmatum thumb. Ethanol Extract Alleviates Interleukin-6-Induced Barrier Dysfunction and Dextran Sodium Sulfate-Induced Colitis by Improving Intestinal Barrier Function and Reducing Inflammation

Figure 2

Effects of KIOM-2015E on Caco-2 monolayer intestinal barrier function. (a and b) Transepithelial electrical resistance (TER). (c) Epithelial paracellular permeability. Intestinal barrier function was analyzed via the TER value, and paracellular permeability was determined using a nonabsorbable, fluorescein isothiocyanate- (FITC-) conjugated dextran probe (FD-4). Caco-2 monolayer cells were pretreated with various concentrations (25–200 μg/ml) of KIOM-2015E for 1 h and then treated with 50 ng/ml IL-6 exposure for 24 h. (d) Representative expression for ZO-1 and occludin proteins. Expression of ZO-1 and occludin in Caco-2 cells was determined by Western blotting. β-Actin was used as the protein-loading control. Densitometry of protein expression. (e) ZO-1. (f) Occludin. The results represent the mean ± standard error of the mean values of three independent experiments. compared with the control group; , , and compared with the IL-6 treated group.

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