Acer palmatum thumb. Ethanol Extract Alleviates Interleukin-6-Induced Barrier Dysfunction and Dextran Sodium Sulfate-Induced Colitis by Improving Intestinal Barrier Function and Reducing Inflammation

Figure 5

The protective effect of KIOM-2015E on tight junctions in the dextran sodium sulfate- (DSS-) induced colitis model. (a) Immunofluorescence staining for ZO-1 expression. Collected intestinal tissues were fixed with 4% paraformaldehyde solution, embedded in paraffin block, and sectioned using a microtome. Histological sections were stained with anti-ZO-1 (red) antibody and DAPI (blue) staining. (b) Representative expression for ZO-1 and occludin proteins. Expression of ZO-1 and occludin in colonic tissues was determined by Western blotting. β-Actin was used as the protein-loading control. (c and d) Densitometry of protein expression. (c) ZO-1. (d) Occludin. These Western blot data expressed the mean ± standard error of the mean values of each mouse in the same group. versus the control group; and versus the DSS-treated group.

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