Acer palmatum thumb. Ethanol Extract Alleviates Interleukin-6-Induced Barrier Dysfunction and Dextran Sodium Sulfate-Induced Colitis by Improving Intestinal Barrier Function and Reducing Inflammation

Figure 6

Effects of KIOM-2015E on macrophage infiltration in the dextran sodium sulfate- (DSS-) induced colitis model. (a) Representative image for the in vivo imaging system (IVIS). (b) Quantification of total photon efflux from a reporter Raw264.7/NF macrophage that migrated into an inflammation site. On the 7th day of the experiment, the mice were injected in the tail intravenously with 100 μl of Raw264.7/NF cells (3 × 106 cells/mouse). On the 8th day of the experiments, the mice were injected intraperitoneally with 100 μl of D-luciferin (3 mg/mouse). The acquisition of BLI intensity measured using IVIS. (c) Immunohistochemistry for F4/80 antibody. Collected intestinal tissues were fixed with 4% paraformaldehyde solution, embedded in paraffin block, and sectioned using a microtome. Histological sections were stained with anti-F4/80 antibody. The total photon efflux results represent the mean ± standard error of the mean values of each mouse in the same group. versus the control group; versus the DSS-treated group.

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