Research Article
Combined Treatment with Zinc Aspartate and Intravenous Immunoglobulins (IVIGs) Ameliorates Experimental Autoimmune Encephalomyelitis (EAE)
Figure 3
Effect of therapeutic application of IVIG, zinc aspartate, and the combination of both on formation of inflammatory lesions in the CNS of EAE mice. EAE was induced by immunization of SJL/J mice with PLP139–151. Mice ( per group) were treated daily i.p. with 10 mg/day IVIG, 30 μg/day zinc aspartate, the combination of both preparations, or vehicle control from day 11 to day 15. Spinal cords were extracted on day 20, fixed in 4% paraformaldehyde, and embedded in paraffin. Sections were stained with H&E. (a) Representative histology of spinal cord longitudinal sections. Inflammatory infiltrations were visualized by H&E staining. The arrowheads show a typical heavy inflammatory cellular infiltration; original magnification ×200. (b) Inflammatory foci in H&E-stained spinal cord cross sections were quantified. Data represent the mean number of inflammatory foci + SEM; (ANOVA).
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