Altered NKp30, NKp46, NKG2D, and DNAM-1 Expression on Circulating NK Cells Is Associated with Tumor Progression in Human Gastric Cancer

Figure 1

Phenotypic analysis of circulating NK cells in GC patients. Human peripheral whole blood from GC patients were stained with anti-CD3, anti-CD56, anti-CD16, anti-NKp30, anti-NKp46, anti-NKG2D, anti-DNAM-1, anti-2B4, anti-CD94, anti-NKG2A, anti-CD158a/h, anti-CD158b, and anti-CD158e1 antibodies or isotype controls. CD3⁻CD56⁺ NK-cell subpopulation was gated, and then, the levels of CD56^high, CD16⁺, NKp30⁺, NKp46⁺, NKG2D⁺, DNAM-1⁺, CD94⁺, 2B4⁺, NKG2A⁺, CD158a/h⁺, CD158b⁺, and CD158e1⁺ cells in NK cells were analyzed. Data were expressed as the mean ± SEM. ; .