TRIF Regulates BIC/miR-155 via the ERK Signaling Pathway to Control the ox-LDL-Induced Macrophage Inflammatory Response

Figure 2

ox-LDL induced miR-155 generation and the macrophage inflammatory response. RAW264.7 cells were treated with 20 μg/mL ox-LDL for the indicated times. (a and b) qPCR was used to detect the expression of miR-155HG (BIC) and miR-155 in macrophages. (c) SOCS1, NFκB p65, STAT3, and p-STAT3 protein expression was evaluated using Western blot. Representative bands show the expression of SOCS1 protein (upper panel), p-STAT3 (second panel), STAT3 (third panel), NFκB p65 (fourth panel), and β-actin protein (lower panel). (d) Histograms illustrated the SOCS1, NFκB p65, and p-STAT3 protein expressions, which were normalized to β-actin expression. The data represented the mean ± S.E. of three independent experiments. (e) The promoter activity of NF-κB was analyzed by dual-luciferase reporter assay. (f, g, h) The expressions of IL-6, TNF-α, and IFN-β mRNA were measured using qPCR. The data represent the mean ± S.E. of four independent experiments. and versus the 0 h time point.

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