TRIF Regulates BIC/miR-155 via the ERK Signaling Pathway to Control the ox-LDL-Induced Macrophage Inflammatory Response

Figure 3

TRIF silencing suppressed BIC and miR-155 expression. (a and b) TRIF mRNA and protein expression was inhibited by TRIF siRNA. Three TRIF siRNA oligos and negative control (NC) were separately transfected into RAW264.7 cells for 24 h, and then, the cells were harvested for detection of TRIF mRNA and protein expressions by qPCR and Western blot, respectively. Representative bands in B show the expression of TRIF protein (upper panel) and β-actin protein (lower panel). (c) Histograms illustrate the TRIF protein expressions, which were normalized to β-actin expression. The data represent the mean ± S.E. of three independent experiments. and versus the NC group. (d and e) BIC RNA and miR-155 expression was evaluated by qPCR. RAW264.7 cells were preincubated with TRIF siRNA-1 or siRNA-2 for 24 h followed by treatment with 20 μg/mL ox-LDL for 24 h. The data represent the mean ± S.E. of four independent experiments. versus the control group and versus the 20 μg/mL ox-LDL/NC group.

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