TRIF Regulates BIC/miR-155 via the ERK Signaling Pathway to Control the ox-LDL-Induced Macrophage Inflammatory Response

Figure 4

Knockdown of TRIF suppressed the miR-155-mediated inflammatory pathway. RAW264.7 cells were preincubated with TRIF siRNA for 24 h followed by treatment with 20 μg/mL ox-LDL for 24 h. (a) The levels of SOCS1 NFκB p65, STAT3, and p-STAT3 protein were evaluated using Western blot. Representative bands showed the levels of SOCS1 protein (upper panel), p-STAT3 (second panel), STAT3 (third panel), NFκB p65 (fourth panel), and β-actin protein (lower panel). (b) Histograms illustrated the SOCS1, NFκB p65, and p-STAT3 protein expressions, which were normalized to β-actin expression. (c) NF-κB promoter activity was analyzed using a dual-luciferase reporter assay. (d and e) The expressions of IL-6 and TNF-α mRNA were measured using qPCR. The data represent the mean ± S.E. of four independent experiments. versus the control group, versus the control group, versus the 20 μg/mL ox-LDL group, and versus the 20 μg/mL ox-LDL/NC group.

(a)

(b)

(c)

(d)

(e)