Research Article
TRIF Regulates BIC/miR-155 via the ERK Signaling Pathway to Control the ox-LDL-Induced Macrophage Inflammatory Response
Figure 5
ERK1/2 is involved in the regulation of BIC and miR-155 expression by TRIF. (a) The expression of p-ERK1/2 protein was evaluated by Western blot. RAW264.7 cells were treated with 20 μg/mL ox-LDL for the indicated times. Representative bands showed the expression of p-ERK1/2 protein (upper panel), p-ERK1/2 protein (middle panel), and β-actin protein (lower panel). (b) Histograms illustrated the p-ERK1/2 protein level, which was normalized to the ERK1/2 level. The data represented the mean ± S.E. of three independent experiments. and versus the 0 h time point. (c) TRIF silencing suppressed p-ERK1/2 expression. RAW264.7 cells were transfected with NC or TRIF siRNA for 24 h followed by treatment with 20 μg/mL ox-LDL for 24 h. Representative bands show the expression of p-ERK1/2 protein (upper panel), p-ERK1/2 protein (middle panel), and β-actin protein (lower panel). (d) Histograms illustrate the p-ERK1/2 protein expression, which was normalized to the ERK1/2 expression. The data represent the mean ± S.E. of three independent experiments. versus the NC group and versus the TRIF siRNA/ox-LDL group. (e) The level of p-ERK1/2 expression was suppressed by SCH772984 (ERK1/2 inhibitor). RAW264.7 cells were preincubated with 1 μM SCH772984 for 2 h followed by treatment with 20 μg/mL ox-LDL for 24 h. (f) Histograms illustrate the p-ERK1/2 protein expression, which was normalized to the ERK1/2 expression. (g and h) The expressions of miR-155 and BIC RNA were measured using qPCR. RAW264.7 cells were preincubated with 1 μM SCH772984 for 2 h followed by treatment with 20 μg/mL ox-LDL for 24 h. The data represent the mean ± S.E. of three independent experiments. versus the control group, versus the 20 μg/mL ox-LDL group, and versus the SCH772984 group.
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