Journal of Immunology Research / 2018 / Article / Fig 6

Research Article

Serum and Ectopic Endometrium from Women with Endometriosis Modulate Macrophage M1/M2 Polarization via the Smad2/Smad3 Pathway

Figure 6

Signaling pathways underlying the macrophage polarization by serum from endometriosis patients. Western blot analysis (a) showed that the protein levels of Smad2 (b) and Smad3 (c) were increased in THP-1 cell-derived macrophages treated with serum from endometriosis patients in the presence of LPS, as compared with the control. By flow cytometric analysis (d), preincubation with SB431542 resulted in a decrease in the percentage of CD163+ cells, which was only observed in macrophages treated with serum from endometriosis patients (e). The mRNA levels of CD86 (f), CD163 (g), IL-12 (h), and IL-10 (i) were detected by RT-PCR. Immunohistochemical staining analysis (j) showed significantly decreased intensity of CD163+ macrophage staining in the presence of serum from endometriosis patients after preincubation with SB431542 (k). LPS: lipopolysaccharide; SB: SB431542. , , .
(a)
(b)
(c)
(d)
(e)
(f)
(g)
(h)
(i)
(j)
(k)

We are committed to sharing findings related to COVID-19 as quickly as possible. We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. Review articles are excluded from this waiver policy. Sign up here as a reviewer to help fast-track new submissions.