The LPS Responsiveness in BN and LEW Rats and Its Severity Are Modulated by the Liver

Figure 2

The G-CSF-induced LPS sensitization was strain dependent. (a) The survival rate after G-CSF pretreatment and LPS administration in LEW rats and BN rats, respectively. (b) The serum AST level was measured 6 h after G-CSF pretreatment and LPS administration. If the rats did not survive for 6 h, the blood samples were harvested at the time of death. (c) Hepatic TNF-α mRNA expression was measured in LEW and BN rats after G-CSF pretreatment and LPS challenge every 10 min within 70 min. Data were shown as mean ± SD ( per group). , (d) The hepatic injury was evaluated by HE staining in LEW and BN rats after G-CSF pretreatment and LPS administration. Representative images from 6 rats per group were selected. (e) The apoptosis was measured by detecting the cleaved levels of caspase 3. The experiments were repeated 3 times, and 1 representative result is shown.

(a)

(b)

(c)

(d)

(e)