Punica granatum L. Leaf Extract Attenuates Lung Inflammation in Mice with Acute Lung Injury

Figure 3

Effect of the pretreatment with EAFPg and dexamethasone on the collagen deposition in lung samples obtained from mice treated with saline (a), LPS (b), 30 mg/kg EAFPg plus LPS (c), 100 mg/kg EAFPg plus LPS (d), 300 mg/kg EAFPg plus LPS (e), or 5 mg/kg dexamethasone plus LPS (f). Quantitative analysis of the collagen area (percentage area) in the bronchial (g) and alveolar parenchyma areas (h). Data is expressed as mean ± SD. Significances were calculated by one-way ANOVA followed by the Newman-Keuls multiple comparison test analysis. differs from saline-treated mice; differs from vehicle-treated LPS-stimulated mice. Collagen areas with leukocyte infiltration are shown as red arrows. Sections (4 μm) were stained with Masson’s trichrome. Magnification of 100x and 400x.

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