STAT5 and TET2 Cooperate to Regulate FOXP3-TSDR Demethylation in CD4⁺ T Cells of Patients with Colorectal Cancer

Figure 2

The interrelationship between STAT5 and TET2 in CRC CD4⁺ T cells. (a, b) Relative STAT5 and TET2 mRNA levels in CD4⁺ T cells from colon tumor tissues () and normal colonic tissues () normalized to GAPDH. (c, left) Representative Western blot results for STAT5 and TET2 in CD4⁺ T cells from colon tumor tissues () and normal colonic tissues (). GAPDH was used for normalization. (c, right) Quantitative analysis of the band intensities for STAT5 and TET2 protein levels normalized by GAPDH. (d) Coimmunoprecipitation using anti-STAT5 in CD4⁺ T cells from colon tumor tissues; detection of the combination of STAT5 and TET2 by Western blot. The same experiments were repeated 3 times. (e) Coimmunoprecipitation using anti-STAT5 in CD4⁺ T cells from colon tumor tissues () and normal colonic tissues (); detection of the binding levels of STAT5 and TET2 by Western blot (, ).

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