Three Types of Broadly Reacting Antibodies against Influenza B Viruses Induced by Vaccination with Seasonal Influenza Viruses

Figure 5

Western blotting analysis of representative Abs against influenza B and A virus strains. (a) Three Abs that showed HI activity. Mouse mAb 3A12 was used as a positive control against influenza B/MAS. (b) Four Abs belonging to type 2. (c, d) Four Abs belonging to type 3. (d) F045-092 and F081-007 were used as a positive control against influenza A/H3N2 and influenza A/H1N1, respectively. (e) Left lane, HA-enriched fraction of virus particles was applied and stained with CBB. Right lane, Western blotting with four mAbs. HA, the position of HA monomer and dimer were clear. However, while HA should be included in the upper bands, it is possible that something other than HA molecule is included.

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