Mesenchymal Stem Cells Ameliorate Hepatic Ischemia/Reperfusion Injury via Inhibition of Neutrophil Recruitment

Figure 2

Rat MSCs reduced the release of liver enzymes and improved the histopathologic changes of livers in IRI. Male SD rats were randomized into sham, IRI + PBS, and IRI + MSC groups. Samples of each group were collected 12 h postreperfusion. (a, b) As markers for hepatic injury, serum levels of ALT and AST were determined. (c) The expression levels of various proapoptotic proteins including Cleaved caspase-3, Bad, and Fas in the I/R liver lobes were determined by Western blot analysis. (d) Representative images of hematoxylin and eosin-stained sections of liver tissues were shown. Inflammatory cell accumulation (solid black triangles), hepatocellular swelling (solid black arrows), necrosis (hollow arrows), and acidophilic degeneration (hollow triangles) were observed. (e) TUNEL staining of apoptosis cells (bright green) in liver tissues. Data are mean ± SD; . Original magnification, ×200.

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