FGFRL1 Promotes Ovarian Cancer Progression by Crosstalk with Hedgehog Signaling

Figure 2

FGFRL1 could be induced by hypoxia via HIF1α in OC. (a) FGFRL1 DNA copy number amplification of OC in TCGA. (b) The mRNA expression level of FGFRL1 was significantly induced after hypoxia treatment in SKOV3 cells in the GSE53012 dataset. (c, d) The protein levels of FGFRL1 and HIF1α stimulated by hypoxia at indicated time intervals in OC cells. (e, f) The protein levels of FGFRL1 and HIF1α under HIF1α siRNA interference and hypoxia condition for 6 hours in OC cells. (g) 10 pairs of primers were constructed according to the promoter of FGFRL1. (h) A ChIP assay was performed to confirm the potential HIF1α binding site in the FGFRL1 promoter region. (i) Two putative HIF1α-binding sites in the FGFRL1 promoter located at −2649 to −2632 and −27 to −11 (mutation site: red). (j, k) A luciferase reporter assay was performed using OC cells after transfecting the wild-type plasmids and mutated plasmids. The data shown are the mean ± SD. ; .

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