miR-34a Regulates Multidrug Resistance via Positively Modulating OAZ2 Signaling in Colon Cancer Cells

Figure 1

Downregulation of miR-34a expression is associated with multidrug resistance in colon cancer (CCa). (a) miR-34a expression in normal colon tissues, primary CCa tissues, and recurrent CCa tissues was determined using RT-qPCR. Fold change was determined for each sample relative to the internal control gene 18S. Each value is a mean ± S.E.M. from three experiments. (b) CCa cells (2.0 × 10⁴/well) were seeded in a 96-well plate. 24 h later, cells were treated with different concentrations of oxaliplatin, paclitaxel, and vincristine as indicated, for another 48 h. Subsequent cell viability was determined using the WST-1 viability assay. The IC50 values (drug concentrations that achieved 50% growth inhibition) were determined as described in Materials and Methods. (c) Characterization of miR-34a expression in different CCa cells using RT-qPCR. (d) Histoculture drug response assay (HDRA) showing the differential chemosensitivities in clinical CCa tissues with different levels of miR-34a expression. and when compared to the Naive cells.

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